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The damage to neurons and synapses is reduced after NES and NES‐DN treatment. (A) Heat maps showing changes in apoptosis and neuron death related genes in WT, MPTP, NES, and NES‐DN. (B) Heat maps showing changes in neural and synaptic related genes in WT, MPTP, NES, and NES‐DN. C, Western blot results of protein levels related to neurons and synapses. (D–G) Quantitative statistical results of changes in neuronal and synaptic related protein levels, NeuN (D), PSD95 <t>(E),</t> <t>synapsin‐1</t> (F), synaptophysin (G). WT ( n = 3), MPTP ( n = 3), NES ( n = 2), and NES‐DN ( n = 3), * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001.
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The damage to neurons and synapses is reduced after NES and NES‐DN treatment. (A) Heat maps showing changes in apoptosis and neuron death related genes in WT, MPTP, NES, and NES‐DN. (B) Heat maps showing changes in neural and synaptic related genes in WT, MPTP, NES, and NES‐DN. C, Western blot results of protein levels related to neurons and synapses. (D–G) Quantitative statistical results of changes in neuronal and synaptic related protein levels, NeuN (D), PSD95 <t>(E),</t> <t>synapsin‐1</t> (F), synaptophysin (G). WT ( n = 3), MPTP ( n = 3), NES ( n = 2), and NES‐DN ( n = 3), * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001.
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The damage to neurons and synapses is reduced after NES and NES‐DN treatment. (A) Heat maps showing changes in apoptosis and neuron death related genes in WT, MPTP, NES, and NES‐DN. (B) Heat maps showing changes in neural and synaptic related genes in WT, MPTP, NES, and NES‐DN. C, Western blot results of protein levels related to neurons and synapses. (D–G) Quantitative statistical results of changes in neuronal and synaptic related protein levels, NeuN (D), PSD95 <t>(E),</t> <t>synapsin‐1</t> (F), synaptophysin (G). WT ( n = 3), MPTP ( n = 3), NES ( n = 2), and NES‐DN ( n = 3), * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001.
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The damage to neurons and synapses is reduced after NES and NES‐DN treatment. (A) Heat maps showing changes in apoptosis and neuron death related genes in WT, MPTP, NES, and NES‐DN. (B) Heat maps showing changes in neural and synaptic related genes in WT, MPTP, NES, and NES‐DN. C, Western blot results of protein levels related to neurons and synapses. (D–G) Quantitative statistical results of changes in neuronal and synaptic related protein levels, NeuN (D), PSD95 <t>(E),</t> <t>synapsin‐1</t> (F), synaptophysin (G). WT ( n = 3), MPTP ( n = 3), NES ( n = 2), and NES‐DN ( n = 3), * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001.
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The damage to neurons and synapses is reduced after NES and NES‐DN treatment. (A) Heat maps showing changes in apoptosis and neuron death related genes in WT, MPTP, NES, and NES‐DN. (B) Heat maps showing changes in neural and synaptic related genes in WT, MPTP, NES, and NES‐DN. C, Western blot results of protein levels related to neurons and synapses. (D–G) Quantitative statistical results of changes in neuronal and synaptic related protein levels, NeuN (D), PSD95 <t>(E),</t> <t>synapsin‐1</t> (F), synaptophysin (G). WT ( n = 3), MPTP ( n = 3), NES ( n = 2), and NES‐DN ( n = 3), * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001.
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The damage to neurons and synapses is reduced after NES and NES‐DN treatment. (A) Heat maps showing changes in apoptosis and neuron death related genes in WT, MPTP, NES, and NES‐DN. (B) Heat maps showing changes in neural and synaptic related genes in WT, MPTP, NES, and NES‐DN. C, Western blot results of protein levels related to neurons and synapses. (D–G) Quantitative statistical results of changes in neuronal and synaptic related protein levels, NeuN (D), PSD95 (E), synapsin‐1 (F), synaptophysin (G). WT ( n = 3), MPTP ( n = 3), NES ( n = 2), and NES‐DN ( n = 3), * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001.

Journal: MedComm

Article Title: Therapeutic Effects of Noninvasive Electrical Stimulation in Combination Transplantation of Human Adipose‐Derived Stem Cells‐Derived Dopaminergic Neuron on the Monkey Model of Parkinson's Disease

doi: 10.1002/mco2.70595

Figure Lengend Snippet: The damage to neurons and synapses is reduced after NES and NES‐DN treatment. (A) Heat maps showing changes in apoptosis and neuron death related genes in WT, MPTP, NES, and NES‐DN. (B) Heat maps showing changes in neural and synaptic related genes in WT, MPTP, NES, and NES‐DN. C, Western blot results of protein levels related to neurons and synapses. (D–G) Quantitative statistical results of changes in neuronal and synaptic related protein levels, NeuN (D), PSD95 (E), synapsin‐1 (F), synaptophysin (G). WT ( n = 3), MPTP ( n = 3), NES ( n = 2), and NES‐DN ( n = 3), * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001.

Article Snippet: Primary antibodies were used: TH (Invitrogen; PA5‐85167), Vinculin (Sigma; MAB3574), GAPDH (Proteintech; 60004‐1‐Ig), MNF1 (abcam; ab126575), MNF2 (CST; 9482S), OPA1 (CST; 80471S), DRP1 (abcam; ab56788), Fis1 (CST; 32525S), NeuN (abcam; ab177487), PSD95 (CST; 2507S), synapsin‐1 (CST; 5297S), synaptophysin (CST; 25056S), Iba1 (abcam; ab178846), GFAP (Invitrogen; 13‐0300), SERPINA3 (Proteintech; 12192‐1‐AP), NF‐kB p65 (CST; 8242S), IL6 (abcam; ab233551), and TNF‐a (CST; 3707S).

Techniques: Western Blot